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Both proteins were purified by His 6 -tag affinity chromatography 62 and SEC on a Superdex 75 HR 26/60 column (GE Healthcare, Munich, Germany) using HEPES-Ringer (135 mM NaCl, 5 mM KCl, 2 mM CaCl 2 , 1 mM MgCl 2 , 10 mM HEPES, 20 mM Glucose, pH 4.7) or phosphate-buffered saline (PBS

When administered, it slows down the process by which the stomach empties its contents into the small intestine

This risk arises because diuretics reduce blood volume